The Need for Improved Diagnostics
It’s also vulnerable to health issues that can place the animal at significant risk, including parasites, ulcers, infection, peritonitis, colitis and, of course, colic.
The SUCCEED Equine Fecal Blood Test changes all of that. With the SUCCEED FBT, veterinarians can obtain reliable objective data about GI tract health. Plus, the Fecal Blood Test can help differentiate foregut from hindgut issues.
The Reality of Equine Ulcers
Most veterinarians are familiar with Equine Gastric Ulcer Syndrome (EGUS) which manifests primarily as lesions in the distal esophagus, the squamous area of the stomach and the proximal duodenum. Gastric ulcers can be visualized with a three-meter endoscope. However, the gastric area represents less than 10% of the equine GI tract.
By contrast, equine digestion is dominated by hindgut action, but ulcers there are much harder to observe. Colonoscopies are impractical due to the difficulty of evacuating the equine colon without endangering the health of the horse. As a consequence, equine practitioners are generally less familiar with colonic ulcers.
The ability to accurately diagnose colonic ulcers and differentiate them from stomach ulceration is of particular importance since the treatment protocols are quite different. At the very least, treatments targeting stomach ulcers are likely to have little or no effect on conditions in the hindgut.
The SUCCEED FBT is an important tool in the veterinarian’s arsenal, providing a source of objective data to support the differential diagnosis of GI tract conditions including gastric and colonic ulcers.
Using Antibodies to Detect Blood Protein Markers
These two protein markers were analyzed using an Enzyme-Linked ImmunoSorbent Assay (ELISA). When the results were plotted, we saw that the levels of hemoglobin peaked and then slowly fell over the 18-hour period, while albumin levels remained consistently low due to the gastric source of the serum. This provided strong support for the utility of these two markers in a differential diagnosis.
From this experiment, we determined that albumin could serve as a proxy for hindgut lesions, while the stability of hemoglobin should allow its use as an indicator of either foregut or hindgut lesions.
For more information, download the Antibody Study (PDF).
The SUCCEED® FBT – An Accurate Diagnostic Aid
There are two wells in the kit, one to detect albumin and one for hemoglobin.
- For albumin, the antibody test was correlated exclusively to the level of colonic ulceration, where the cutoff was set to grade 1 and above.
- For hemoglobin, the test correlated well to the overall level of observed GI ulceration when the positive gastric and colonic cutoff was set to grade 2 and above.
|Positive Predictive Value||95.4%||96.9%|
|Negative Predictive Value||75.0%||57.9%|