How to Use the SUCCEED® Equine Fecal Blood Test

The SUCCEED FBT tells you what you need to know, on the spot. Results are available in 15 minutes at the most – but generally less. No expensive equipment, no hauling, and no tricky, time-consuming, or invasive procedures, no waiting hours or even days for results. By running the FBT, veterinarians can determine if additional, more complex, diagnostic methods are necessary, or may be armed with the information needed to make an initial diagnosis and begin treatment.

Simple Step-by-Step Instructions for Use

The SUCCEED FBT is easy to administer and requires no special training or skills to use. Here are the step-by-step instructions requiring just the contents of the kit, clean tap water, and a fresh  fecal sample:

  • Collect a fresh (less than 4 hours old) fecal sample from the subject horse, loosely dropping fecal matter into the provided plastic container up to the Sample Fill Line.
  • Add clean tap water (potable, room temperature), filling to the Water Fill Line on the container.
  • Replace lid on container and shake thoroughly to ensure complete mixing.
  • Use the provided pipette to collect some of the fecal/water mixture from the container, avoiding particles.
  • Apply two drops of solution to each of the two sample wells on the test cassette.
  • Test results should appear within 15 minutes.

Watch this instructional video to see these steps in action:

Click here to download the full FBT Instruction Sheet (PDF).

How to Know the FBT is Working

The dual cassette includes two red, horizontal line indicators each for Test A and Test H. The first is a control line that shows the test is working; this should appear every time you run the test. The second is the test line, which only only appears if the test is positive. The FBT is sensitive to heat and must be refrigerated when not in use. Be sure to read and follow the storage and handling instructions to ensure the FBT will work properly.

Reading the Equine Fecal Blood Test Results

While the test itself is easy to use, understanding what to do with the results in light of a full diagnostic work-up requires the skills and knowledge of a trained and certified veterinarian. 

  • If a solid line appears only at the CTL point (Control line) on either Test A or Test H, this is a negative result.
  • If a complete, solid line appears at the TST point (Test line) on either or both Test A and Test H, that is a positive test result.

The potential combinations of positives and/or negative results for the blood components albumin (Test A) and hemoglobin (Test H) can indicate issues in the foregut, hindgut, or both, and may provide some indication of the severity of the source of bleeding. This information may be used to provide objective insight into the full evaluation provided by veterinarians. Learn more about what positive and negative test results indicate as well as how to understand these results in light of a full diagnostic work-up.